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| 变形链球菌表面蛋白真核表达载体pcDNA3—PAc的构建
idv8。cin皓hthepreventionofin~eetiousdi3eaVaccine，1997,converse pas cher，15f12)：Ⅶf1999—07—91收幡)Constructionof5lreptococcusmutansSurfaceProteinAntigenEukaryoticExpressionVectorpcDNA3一PAc1．ExtractionandPurificationofPlasmidDNApPC41andpcDNA3IiuJianguo，LiuTianiia,tiffany deutschland，ZhouXuedong,moncler uomo。eta1CollegeofStomototog5，CUniversityofMedicalSciencesIiuIi．JiaWenxlangCtdlegeofBasicMedicalSciences，WestChinaUniversityofMedicalSciencesAbsttactObjective=ToExtractandpuffy山smidDNApPC41andpeDNA3．Methods：Bacteriaweree~leetedandlyzedbytheaIka．1lnelysismethod-ThephsraidpPC41carryings~ucturalgenepecandcloneexpt~oaatontorPeDNA3wereexhaledandDl．fledfromE．coliclonehythepdyethylenegl~-olpredpitatlngmethod,tory burch shoes，theelectroelutlonintodialy3bagsmethod，thelowmelt，ing-temperatureagaroaegelandgIa∞fiber~lumnchromatographyThecOnCentration，purityandmoheularweightwedeter-minedbyspeetrophotometry，restr；ctionenzymeandagaro~gelelectroph~eais．Ruuft$Theco~~entratkmofplasmidDNAofthisexperimentingeneralwss0·12～0，24g／L，andA／A∞ofthepolyethyleney0。1precipitatingm~hod，theeleetroelutlonintodialysisbagsmethod，thelowmetllng-temperatureagr~segelandglassfibercolumnchrornatograhywtreect；、b1．9，2·2．2．2aI1d2-6．Molecularwe；ghtofpPC41andpeDNA3Wa日~．．o,peetively10．6khaI1d5．4kh．Cond：Thefourmeth,belstaff uomo，odscanobtainpurephsm~DNA·Purltyofpl~smidDNAobtainedbygbfibercdum．chrornatographymethodisthehighestinthefourmethods·G1afibercolumnchromatographyiso#leoftheeffectivemethodsgettinghighlypurifiedplasmidDNAKeywofulstreptococcus搠d描surfaceproteinantigenplaamidDNApurification More articles related to topics： GLUMA、极固宁及氟化钠甘油治疗牙齿敏感症的疗效观察_1851 Mont Blanc pens 吉西他滨为主治疗肺转移癌4例报告_18 moncler outlet 氯胺酮复合异丙酚静脉麻醉在小儿斜疝 |
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